The Mitchell laboratory welcomes Andrew Bell to the lab! Andrew is a part of the KURE (Kidney Undergraduate Research Experience) program, an NIH funded Summer Research Program!
The Mitchell laboratory welcomes Dr. Parveen Kumar, PhD to the lab!
Oxalate induces mitochondrial dysfunction and disrupts redox homeostasis in a human monocyte derived cell line
Monocytes/macrophages are thought to be recruited to the renal interstitium during calcium oxalate (CaOx) kidney stone disease for crystal clearance. Mitochondria play an important role in monocyte function during the immune response. We recently determined that monocytes in patients with CaOx kidney stones have decreased mitochondrial function compared to healthy subjects. The objective of this study was to determine whether oxalate, a major constituent found in CaOx kidney stones, alters cell viability, mitochondrial function, and redox homeostasis in THP-1 cells, a human derived monocyte cell line. THP-1 cells were treated with varying concentrations of CaOx crystals (insoluble form) or sodium oxalate (NaOx; soluble form) for 24 h. In addition, the effect of calcium phosphate (CaP) and cystine crystals was tested. CaOx crystals decreased cell viability and induced mitochondrial dysfunction and redox imbalance in THP-1 cells compared to control cells. However, NaOx only caused mitochondrial damage and redox imbalance in THP-1 cells. In contrast, both CaP and cystine crystals did not affect THP-1 cells. Separate experiments showed that elevated oxalate also induced mitochondrial dysfunction in primary monocytes from healthy subjects. These findings suggest that oxalate may play an important role in monocyte mitochondrial dysfunction in CaOx kidney stone disease.
Congratulations to Adam for being awarded “The Gateway to Research Award” from the American Foundation for Pharmaceutical Education with Dr. Robert Wang from Samford University McWhorter School of Pharmacy! Adam is the first student to receive this award at Samford!
The Mitchell Lab welcomes, Glory Akinduro, a UAB-Genentech Undergraduate Research Scholar for the summer!
The Mitchell laboratory welcomes Dr. Mikita Patel, MD to the lab!
Dr. Mitchell was awarded a NIH NIDDK K01 Mentored Scientist Award for 5 years! She is the first person to receive this award in her department!